|
棉 花 学 报 Cotton Science 2008，20(3)：179-185
Molecular Marker and QTL of Yield-related Traits in Transgenic Insect-resistant Cotton Varieties Abstract：The cross of sGK9708×0-153 was made, which included one parent with high fiber strength,0-153,originating from crossing of G.hirsutum L. with G.arboreum L., and backcrossing with G.hirsutum L. as the recurrent parent, and the other commercial transgenic variety with resistance to budworm, sGK9708. A total of 3869 pair SSR primers were used to screen polymorphism among two parents and their F1, which resulted in 150 polymorphic loci from 125 pair primers in F2 population. Linkage test indicated that 100 loci could be mapped to 20 linkage groups, which covered a total genetic distance of 660 cM, approximately 14.67% of cotton genome. The average distance of neighbor markers was 6.6 cM. 13 linkage groups were assigned to corresponding chromosome, respectively. Based on Composite Interval Mapping (CIM) method, the result showed that 28 QTLs for fiber yield related traits were detected in F2and F2：3 populations. These QTLs of yield-related trait existed in the five linkage groups only, clustered on the same locus in different chromosomes. Two QTLs of lint yield clustered with many QTLs of other yield related traits in two different linkage group segments with same direction of genetic effects. So it is necessary to study the effect of the marker-assisted selection for the two QTLs in the future. No same QTL for the yield-related trait could be detected in different generations, which shows multiple self-crossing lines should be developed for QTL analysis of yield-related traits.
|