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棉 花 学 报 Cotton Science 2008，20(3)：174-178
Study on a New Rapid Inoculation Method for Verticillium wilt and Fusarium wilt of Cotton Abstract：A rapid and effective inoculation method is the basic technique for identifying cotton varieties'resistance to Verticillium wilt and/or Fusarium wilt and differentiating the pathogenicity of these pathogens. This study was carried out to search a new inoculation method with high efficiency for cotton Verticillium wilt and/or Fusarium wilt. The experiment was developed in the field and the greenhouse whose temperature and light was adjustable. Three non-injured cotton root inoculation methods: the whole stage field disease plot appraisal, dipping cotton seedling roots cultured in liquid with spore suspension, and irrigation cotton seedling roots cultured in soil with spore suspension,were compared for their effectiveness in infection and development of Verticillium wilt and/or Fusarium wilt of cotton. Results showed that, among the three inoculation methods, entire test period with method of field disease plot appraisal needed six months or more, method of irrigation cotton seedling roots cultured in soil with spore suspension needed two months, but dipping cotton seedling roots cultured in liquid with spore suspension (the inoculation concentration was 107 conidia·mL-1) needed only 30 days. Besides,by the third inoculation method, not only the cotton varieties ' resistance to Verticillium wilt and/or Fusarium wilt, but also the strains ' pathogenicity of Verticillium dahliae and/or Fusarium oxysporum f.sp. Vasinfectum could be appraised. The result was accorded with the field appraisal. Furthermore, for this method need not injure any parts of the plant,so it can make the result more accurate and the plants were suitable for the quite precise research. The different inoculation times （20min，30min，40min，60min）were also optimized, and the results were showed that the inoculation time lasted for 40 min was the most optimum and got the consistent results with that by field disease plot. Dipping cotton seedling roots cultured in liquid with spore suspension is a rapid, reliable and steady inoculation method for Verticillium wilt and/or Fusarium wilt of cotton. Presently, improvement of this method is tried to use in the screening of cotton mutant for resistance to Verticillium wilt and/or Fusarium wilt and in the testing of function for pathogenicity genes.
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