棉 花 学 报      Cotton Science    2008，20(2)：110-115

Study on Role of Foreign Cellulose Synthase Genes in Improvement of Cotton Fiber Quality
ZHANG Hai-ping^1,2，WANG Xue-de¹，SHAO Ming-yan¹，YUAN Shu-na¹，LI Xiao¹
（1．Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China；2．Institute of Crop Germplasm Resources of Shan xi Academy of Agricultural Sciences, Taiyuan 030031, China）

Abstract：In order to improve cotton fiber quality, the foreign cellulose synthase genes (acsA and acsB ) which were from Acetobacter xylinum , driven by CaMV35S promoter, were introduced into brown color cotton G007, and white color cotton X003, by two means of pollen tube-mediated transformation (ovary injection and pollen grain mediation). Ovary injection, compared to pollen grain mediation, resulted in higher transformation frequencies. Intergration of acsA and acsB genes was confirmed by PCR and southern blot, the result indicated that 11 plants possessed acsA and acsB genes. Four plants with better agronomic traits and fiber quality were selected from transformants. Analysis of transgenic plants and their progenies indicated that some of their fiber properties were improved. The length, strength, cellulose content and lint percent of brown color cotton fiber were increased significantly. However, for white color cotton, although the strength and cellulose content of fiber were increased significantly, the length and lint percent weren ' t changed. The fact suggested that induction and expression of acsA and acsB improved fiber quality of transgenic cotton plants.
Key words：Cotton (Gossypium hirsutum L); Cellulose-synthase gene; Fiber quality improvement；Gene transformation            [Full Text, 2770KB]