棉 花 学 报 Cotton Science 2007，19(4)：261-266
Cotton Genetic Transformation of Barnase Male Sterility Gene Abstract：Cotton male sterility mutations naturally have been proved to be useful for producing hybrids. Crosses between cotton lines often result in progeny with higher hybrid vigor. However，the hybrid production has been limited because there is no ideal fertility restoration variety. The establishment of genetic engineering strategy of male sterility and fertility restoration varietyies has facilitated the development of breeding system for hybrid cotton. To obtain genetic modified male sterile cotton，the chimaeric male sterile gene which was constructed via placing the ribonuclease barnase under the control of anther-specific promoter was used to transform hypocotyledonary axis of cotton via Agribectrum tumefaciens . Embryoids were generated from transformed calli and then developed to seedling. 150 mg·L－1 Kanamycin was used for selecting positive transgenic seedling from the primary one from embryoids and many negative transgenic seedlings were screened out. 120 lines of transgenic male sterility were identified via PCR and Southern dot blotting. 69 lines of the identified transgenic plants were conferred a resistance to bromoxynil and male-sterile traits，however，only 6 lines showed significantly male-sterile traits.
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