棉 花 学 报     Cotton Science   2006,18(6):338-341

 

Studies on the Effects of the Salinity Priming on the NaCl Tolerance of Transgenic Insect Resistant Cotton ( Gossypium hirsutum L.)
LIN Jun,SUN Yu-qiang,Lü You-jun,ZHU Shui-jin*
(Agronomy DepartmentCollege of Biotechnology and AgricultureZhejiang UniversityHangzhouZhejiang 310029,China )

Abstract:The effect of seed priming with salts on the seed germination and seedling emergence of the transgenic insect resistant cotton stressed by NaCl was studied in this paper,using two extending transgenic insect resistant cotton cultivars,CCRI 41 and Z-905,as the materials and their genetic background cultivar,CCRI 23,as check. The results indicated that it was harmful for the seed germination and field emergence of the upland cotton seeds when they were treated by 0.5% NaCl,especially for the transgenic insect resistant cotton cultivars whose germination percentage and field emergence was much lower than that of their genetic background check. However,priming treatment with a certain concentration salts could alleviate the harmful of the NaCl stress significantly. After the treatment of seed priming, the germination percentage and seedling emergence on the condition of 0.5% NaCl stress increased significantly,and the seed tolerance to the salt stress enhanced greatly. There were some differences in the effect of the seed priming treatments on the seed germination and field emergence,stressed by NaCl,between the transgenic insect resistant cotton and their genetic background one. The formers,with weak tolerance against NaCl stress,had a better effect than that of their genetic background one without exogenous insect resistant genes. There were some differences among the kinds of the priming agents,and K2SO4 was the best one in this experiment. In addition,there were some interaction between the concentration of the priming agents and the treatment time. The treatment time should be longer for the priming agents with lower concentration than that with higher concentration.
Key words:salinity stress;transgenic insect resistant cotton;seed priming;seed germination  
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