棉花学报   Cotton Science  2006,18(1):3~7

 

The Effects of Mixture of DPC and DTA—6 on Seedlings of Insectresistant Transgenic Cotton
TIAN Xiao-li,TAN Wei-ming,LI Zhao-hu,WANG Bao-min,HE Zhong-pei,DUAN Liu-sheng
(Center of Crop Chemical Control,College of Agriculture & Biotechnology,China Agricultural University,Beijing 100094,China)

Abstract Most insect-resistant transgenic cotton developed slowly in North China Plain, which made it susceptible to plant growth retardant DPC. The objective of this research was to verify the effects of mixture of DPC and plant growth accelerant DTA-6 (diethyl aminoethyl hexanoate) on seedling development of insect-resistant transgenic cotton and to offer data for developing new plant growth regulator fitting to it. Compared with single application of DPC,the mixture of DPC and DTA-6 not only increased the root length and the number of lateral root primordia of insect-resistant transgenic cotton,but also significantly diminished the inhibition of up-ground part caused by DPC. For example,the hypocotyls length of CCRI 30 decreased about 3% only,and the leaf area of seedlings of CCRI 30,DP99B and sGK321 was all equivalent to 93%~94% of respective control. These results were attributed to the increase of IAA concentration. in top, cotyledon and hypocotyls of insect-resistant transgenic cotton. Furthermore,the mixture of DPC and DTA-6 increased the number of granalamellae in chloroplast and enhanced the photosynthetic rate of functional leaf of insect-resistant transgenic cotton. Obviously,the mixture of DPC and DTA-6 retained the function of enhancing root development of DPC,which was in favor of adversity-resistance and preventing premature senescence. At the same time,DTA-6 counteracted the inhibition of up-ground part caused by DPC in great extent,which was in favor of seedling rapid growth of insect-resistant transgenic cotton.
Key wordsDPC;DTA-6;mixture;insect-resistant transgenic cotton;growth and development
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