Cotton Science 2005£¬17(5)£º269-274
Inheritance and AFLP Marker of Resistance in Helicoverpa armigera Abstract: The results of resistance genetical analysis showed that the Cry1Ac toxin resistance in resistant strain H.armigera selected by transgenic Cry1Ac cotton was incompletely recessive, and the backcross progeny segregated 1¡Ã1£¨X2< X20.05£© for resistant vs. susceptible phenotypes, feeding diet with 40 ¦Ìg¡¤ml-1 Cry1Ac for 5 d. AFLP method was applied to map the resistant gene marker in H.armigera to transgenic Cry1Ac cotton. The results showed that 82 DNA markers were generated from 47 BC1 progenies by ten primer combinations, among which 12 markers were distributed into four linkage groups; BtR gene flanked with two AFLP marker, EaaMcta02 and EaaMcta03, on the 2nd linkage group in H.armigera, with 10.40 cM and 13.90 cM linkage distance; meanwhile, 68 markers were gained from 48 BC2 progenies with the same primer combinations, among which 29 markers clustered into nine linkage groups, and BtR gene couldn't be located on any group. The results also suggested that the number of amplified fragments depends on the primer combination and usually ranges in number from 50 to 100, and AFLP amplification product can be visualized by silver staining with sensitivity and resolution similar to these observed with radioactive labelling. The results provide a guidance in understanding, monitoring, and managing resistance to transgenic Bt cotton in H.armigera.
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