Cotton Science 2005£¬17(5)£º264-268
Studies on Method of Inducing Cotton Multi-Resistance to Wilt Diseases by Artificial Inoculation in Breeding Abstract: The strong virulence pathogen strains of wilt diseases were cultured in autoclaved cottonseed to make pathogen carrier respectively. Fusarium oxysporum f.sp.Vasinfectum was inoculated with cottonseed meal at the dosage of 90 g¡¤m-2 in nursery soil before sowing. Then each seedling was inoculated with 10 ml of Verticillium dahliae spore suspension at the concentration of 2¡Á107 spores per milliliter by watering injured roots at 2 true leaf growth stage. The nursery was covered with polythene film to keep the average bed temperature of 20¡«25¡æ. Thus the wilt diseases symptoms would appear on susceptible plant within 15¡«20 days after inoculation of Verticillium dahliae spore suspension. The varieties, of which Fusarium wilt disease incidence was over 8% or Verticillium wilt was over 12%, and the susceptible plants of resistant variety were eliminated. Then the resistant plants of resistant varieties were selected and transplanted to the field. It had been proved that this method of inducing cotton multiª²resistance to wilt diseases by selection with artificial inoculation was effective and practicable. With applying of this method, several cotton resistant varieties had been bred.ª¤
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