Cotton Science 2005,17(4):240-244
Advances in Cotton Gene Cloning Abstract: Advances have been obtained in the research of cotton gene cloning, which is mainly related to fiber quality, stress resistance, genes related gossypol, chloroplast genes, regulation elements, and enzymes, which are related to growth. In China, genes cloned in cotton fiber quality included β-canaliculus protein, E6gene, GhbZIP,GhIAAl6 and about forty cotton-fiber special expression genes. Abroad,Fbl2A 、Rac13、Rac9、MYB gene and about twenty fiber development genes have been separated.Several genes were cloned in the field of stress resistance, such as LRR-RC gene,NBS-LRR,RGAs,14-3-3 protein and PR protein gene and some cDNA clones, and in the field of regulation elements，Lea-113，LIM domain gene，α-receptor protein gene and LTP3 promoter, CAP gene,Fb-B6,FbL2A promoter,GhTUB1 promoter. As for chloroplast genes, we have gotten rps7,ndhB,a/b bindingprotein gene, RUBP carboxylase gene, NADH dehydrogenase gene, et al, and in the field of enzyme, genes cloned were FPS,FPP,C4H,GhCtp and CP, et al; in the field of cotton genes related gossypol, only two genes were obtained which were cotton (+)-δ-cadinene synthase gene and desoxyhemigossypol-6-o-methyltransferase gene. Cloning method included: cDNA liberty, genome liberty and RACE, et al.
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