棉 花 学 报  Cotton Science 2005，17(3)：131-136

Synchronous Expression of the CryIAc and CpTI Genes in Transgenic Cotton
KANG Bao-shan^1，2 ，ZHANG Rui¹，PAN Deng-kui³，WANG Yuan^l，GUO San-dui^l
(1. Biotechnology Research Institute，Chinese Academy of Agriculture Sciences，Beijing 100081；2. Zhengzhou Fruit Research Institute，Chinese Academy of Agriculture Sciences，Zhengzhou 450009，China；3. Institute Arts and Science，Shanxi Agriculture University，Taigu 030801，China)

Abstract：PCR，Southern blotting were usedto analyze the characteristics of Cry1Ac gene and CpTI gene in transgenic cotton genome and the cotton derived in eight different transgenic cotton harboring double-gene varieties or lines through pollen-tube transformation. The results demonstrated that the copy number and insertion site of the same gene in eight varieties or lines are not consistent completely. The insert sites of CrylAc and CpTI of the same varietie or line are different except sGK321 and 3517，moreover，only one insertion site is uniform in sGK321. In the meantime， it was found that one blotting signal was around the position of 4.7 kb in all southern blotting maps of Cry1Ac gene. ELISA measured the two proteins of Cry1Ac and CpTI concentrations in different varieties or lines. As a result，the two genes of Cry1Ac and CpTI cannot express synchronously in the same line and position. Their expression exist much difference. And the variations were unstable at the whole development stage. These results indicated that two series of foreign genes， Cry1Ac gene and CpTI gene，on plasmid pGBI121S4ABC were not absolutely linkage integration and synchronous expression in most of transgenic plant through pollen-tube transformation.
Key words: pollen-tube pathway transformation；transgenic cotton habouring double-gene；
            Cry1Ac gene，CpTI gene；synchronous expression    [Full Text, 3157KB]