Cotton Science 2005,17(2):72-78
Identification of Aphis£¨Aphis gossypii Glover.£©Resistant of Cotton(Gossypium hirsutum L.) with Transª²exogenous Agglutinin Gene Abstract: During aphis occurring period, aphid-resistant character can express stably in engineering plants of trans-exogenous agglutinin gene cotton. The segregation proportion of the first transformed generation population accords with 3¡Ã1, the aphis-resistant gene is integrated into cotton genome with single locus. New varieties of transgenic cotton resistant to aphis can be obtained by successive with identification, selfing and selection through four generations. With the experimental materials of three cotton varieties with trans-exogenous agglutinin gene, two morphological aphis-resistant cultivars and six conventional cultivars, identification on transgenic cotton resistant to aphis are dealt with in this study. The results indicated that three transgenic varieties are highly resistant to aphis, Chuankang 77 is in the middle level of resistance to aphis during growth and development periods, Chuanmian 109 is sensitive to aphis in seedling stage and recovery stage, but expresses middle resistance in midsummer stage, six conventional cultivars are highly susceptible. The results are same through three different kinds of aphis transferred pathway using two different kinds of varieties resistant to aphis, one being transgenic, the other being morphological. Identification on transgenic cotton resistance to aphis in net-room overcomes randomicity of aphis distribution in field identification and limitation of small acreage in greenhouse identification, with better repeat and higher differentiation than others. Natural immigration between its reproductive rows is a convenient and high efficiency pathway for aphis transference in resistance identification, because the program of aphis manual transference was omitted in this pathway. |
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