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棉 花 学 报 Cotton Science 2005，17(1)：12-17
Establishment of Cotton Embryogenic Suspension Cultures and Abstract:Systematic researches on the initial embryogenic callus, the combinations of plant hormones, the components of the media, the cells' growth curve and the cells' senescence during suspension culture reveal that the media MS+2,4-D 0.5 mg·L-1+KT 0.1 mg·L-1and MS+2,4-D 0.1 mg·L -1+KT 0.1 mg·L-1 were suitable for cells proliferation and could produce uniform cells and small cell aggregates with strong viability which were optimal population to study cell growth and its regulation, while the media MS+IBA 0.5 mg·L-1 +KT0.1mg·L-1 and MS+IBA 0.1 mg·L-1 +KT 0.1 mg·L-1 were fit for producing big cell aggregates and embryoes of different developmental stages which could be used to induce and gather embryoes. K+and inositol were important chemicals for cell proliferation but suppressed somatic embryogenesis of the suspension culture, which could be used to improve and adjust cell growth patterns and viability. In the course of suspension, the fistular cells were substituted by globular cells and small cell aggregates. The growth curve of suspension cells was typical of“S”. In the first three days, the cells grew very slowly, from 3 to 15 days the cells proliferated exponentially, and 15 days later the cells proliferation was slowed down. So the suspension culture should be subcultured before the 15th day to sustain the strong cell division competence and cell viability.
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