15 July 2016, Volume 28 Issue 4
    

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  • Xia Bing, Ren Xiaoming, Du Mingwei, Xu Dongyong, Yin Xiaofang, Tian Xiaoli, Li Zhaohu
    Cotton Science. 2016, 28(4): 315-323. https://doi.org/10.11963/issn.1002-7807.201604001
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    High plant populations and multiple applications of the plant growth retardant mepiquat chloride (MC) are required in the production of mechanically harvested cotton (monocropping) in the Yellow River Valley. Cotton-stalk recycling to the field is one of the main approaches to avoid the further increase in fertilizers during cotton production. However, the optimum nitrogen (N) rate and whether N use efficiency would increase under these conditions remains unclear. We conducted experiments involving cotton-stalk recycling to the field at Hejian in Hebei Province (38°41′N, 116°09′E, and 11 m elevation) during the 2013 and 2014 growing seasons. In 2013, there was a considerable amount of precipitation (279.1 mm) in July (flowering period); however, in contrast, there was only 42.4 mm precipitation in the same month of 2014. We investigated the effects of plant density, MC application and N rate on cotton yield and its components, accumulation and distribution of biomass and N, as well as N use efficiency. In comparison with the low plant density treatment (6.75 × 104 plant·hm-2, LPD), the seed cotton yield and Npartial factor productivity (PFPN) under the high plant density treatment (11.25 × 104 plant·hm-2, HPD) significantly increased by 8.1% and 7.4% in the drier year (2014). In addition, the N apparent recovery efficiency (REN) of HPD also significantly in- creased to 41.6% in 2014. The MC application significantly increased the seed cotton yield (39.2%), PFPN (43.3%), and agronomic efficiency (212.8%) relative to the water control (CK) in the wetter year (2013). The REN of MC was also greater than that of CK in 2013 but this difference was not significant. The HPD showed increased dry matter, but a decreased harvest index (HI) in 2013. Although less dry matter was produced, the MC increased HI in both the wetter and drier years. The N rate ranged from 0 to 315 kg·hm-2 did not significantly affect the seed cotton yield, but there was only a tendency that the low N rate (105 kg·hm-2, LN) had a higher yield compared with the medium (210 kg·hm-2, MN) and high N rates (315 kg·hm-2, HN). Therefore, the LN treatment had the highest PFPN, which was 24.5 and 54.4 kg·kg-1 in 2013 and 2014, respectively. The REN of LN was 45.2% and 41.0% in 2013 and 2014, also significantly greater than those of MN and HN. In summary, the combination of HPD and MC application was in favor of the biomass accumulation and HI increase in addition to the yield stability of mechanically harvested cotton in the Yellow River Valley, and the optimum N rate could be reduced to 105 kg·hm-2 when stalks are returned to the field.
  • Ma Hui, Wang Qi, Zhao Ming, Wang Hongyan, Ji Xianglong, Dong Hezhong
    Cotton Science. 2016, 28(4): 324-330. https://doi.org/10.11963/issn.1002-7807.201604002
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    Abiotic stresses such as salinity and drought affect the secondary metabolism in cotton plants, which may further influence the occurrence of pests on the stressed plants. To determine the relationships of abiotic stress with secondary metabolism and cotton aphid population dynamics, we conducted an experiment to determine the gossypol and soluble sugar contents in cotton leaves stressed with different concentrations of NaCl and PEG (polyethylene glycol), respectively. The development and multiplication of cotton aphids on cotton seedlings were also studied. The results indicated that the gossypol contents in cotton leaves increased by 20% and 53.5% with 100 and 200 mmol·L-1 of added NaCl, respectively. The soluble sugar contents increased by 15.7% and 46%. The developmental period of cotton aphids in salt-stressed plants was significantly extended, while the reproductive rate of cotton aphids was significantly reduced. The durations of the nymph stage under the 100 and 200 mmol·L-1 NaCl treatments increased by 6.4% and 9.9%, relative to the control, respectively; The fecundity of the single female reduced by 22.6% and 52.3%, respectively; Accumulation of the gossypol and soluble sugar in cotton leaves was also enhanced by drought stress; The gossypol contents increased by 39.7% and 61.5%, and the soluble sugar contents increased by 14.2% and 47.1%, respectively. The durations of nymph stage of 2.5% (mass fraction) and 5% PEG treatments increased by 7.4% and 16.5%, relative to the control, respectively; The fecundity of the single female reduced by 26.8% and 55.8%, respectively. Correlation analysis indicated that the gossypol and soluble sugar contents in cotton leaves were positively correlated with the duration of the nymph stage and negatively correlated with the cumulative fecundity. Thus, salt stress enhanced secondary metabolism and increased accumulation of secondary metabolites such as gossypol and soluble sugar, which finally inhibited the population dynamics of cotton aphids.
  • Yang Changqin, Zhang Guowei, Liu Ruixian, Zhang Lei, Zhou Guanyin
    Cotton Science. 2016, 28(4): 331-338. https://doi.org/10.11963/issn.1002-7807.201604003
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    Using the early mature cotton (CCRI 50) as material, a field experiment was carried out to evaluate the effects of the plant growth regulator mepiquat chloride (DPC) on yield and canopy architecture of cotton sown after harvesting barley in Nanjing, Jiangsu Province, China, in 2013―2014. A split-plot design with three replicates was used for the study. The main plots comprised three planting densities (7.5 × 104, 9.75 × 104, and 12 × 104 plant·hm-2), while different DPC application rates (0, 52.5, and 105 g·hm-2) constituted the subplots. The results showed that the lowest lint yields were found at the planting density of 12 × 104 plant·hm-2 and the DPC addition rate of 0 g·hm-2, respectively. A significant interaction was detected between planting density and DPC on lint yield and as a result, the treatment of 9.75 × 104 plant·hm-2 with DPC of 52.5―105 g·hm-2 achieved higher lint yield than other treatments. The boll number of yield component has the largest direct effect on lint yield. In terms of canopy architecture, the angles and length of low fruit branches decreased with planting density increase, while the larger angles, length, and leaf area index of middle and upper fruit branches were found at 9.75 × 104 plant·hm-2. The angles and length of fruit branches and the leaf area index decreased with the DPC application rate increase but the opposite was observed for the transmittance. The correlation analysis between the canopy characteristics and lint yield indicated that it was conducive to increase the yield and seed cotton rate before frost for plants with larger angles at the low fruit branch, longer length of the middle fruit branch, and smaller angles at the upper fruit branch with a higher leaf area index and transmittance. In summary, cotton sown after harvesting barley at a planting density of 9.75 × 104 plant·hm-2 with DPC of 52.5―105 g·hm-2, which was applied at the ratio of 1∶2∶4 at the budding stage, flowering stage, and after topping, could improve canopy architectures, thus increasing earliness and yield of cotton in the lower reaches of the Yangtze River.
  • Cui Bibo, Han Yong, Wang Weiyi, Ji Ronglong, Sun Kouzhong, Li Yafang, Geng Anhong, Fei Yueyue, Wang Jun, Peng Yamin, Chen Dehua
    Cotton Science. 2016, 28(4): 339-344. https://doi.org/10.11963/issn.1002-7807.201604004
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    With cotton cultivar CCRI 79 as the experimental material, six treatments (high ridge with mulching film, high ridge, soil desalting agent with mulching film, soil desalting agent with both high-ridge and mulching film, soil desalting with high-ridge, and conventional seeding with mulching (control)) were designed to investigate emergence, yield, and physiological features related to salt resistance in the moderate to severe saline soil of the Dafeng Rice and Wheat Farm in Jiangsu Province in 2012 and 2013. The results showed that the treatment soil desalting agent with both high ridge and mulching film markedly improved seedling establishment and yield compared with the control; the seed cotton yield increased by 32.00%―113.78%, the soil salinity contents decreased by 42.07% at the 0―20 cm soil layer, and the emergence rate increased by 148.8%. Furthermore, the treatment decreased the leaf malondialdehyde and proline contents effectively, and reduced leaf superoxide dismutase activities were also observed. These results suggest that soil desalting agent application with both high ridge and mulching film reduces the salinity content of moderate and severe coastal saline soil, which benefits seedling establishment, decreasing the effect of the salt stress on the growth of cotton plant, and thus enhancing the seed cotton yield.
  • Gao Lili, Li Gan, Kang Zhenghua, Li Jianwei, Wang Mifeng, Ma Yunzhen, Zhang Jusong
    Cotton Science. 2016, 28(4): 345-352. https://doi.org/10.11963/issn.1002-7807.201604005
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    To explore the physiological mechanism of defoliating cotton leaves under different spraying times, we investigated the absorption, transmittance, and transformation of solar radiation by cotton leaves using the chlorophyll fluorescence determination method. Results show that the applied defoliant reduced cotton leaf photosynthesis, maximum photochemical efficiency, photosynthetic electron transport rate and photosynthetic quantum yield, but increased minimal fluorescence and dissipation of non-radiant energy, which indicates that cotton leaves might experience some degree of photosynthetic protection under defoliant stress. Compared with thidiazuron, Dropp Ultra (Bayer, Germany) is more effective in accelerating the process of cotton leaf senescence. When a defoliant was applied to cotton when the boll opening rate reached 10%, boll weight, lint percentage, and lint yield were significantly lower than the contro (spraying water)l. When spraying the plants with Dropp Ultra 300 g·hm-2 + 40% ethylene 1200 mL·hm-2 when the boll opening rate reached 50%, the rate of shed leaves was 90.1% and the boll opening rate was 99.87% after a period of 25 days, which indicates this application has minimal impact on cotton boll weight, lint percentage, and lint yield, and is more conducive to the promotion of mature bolls and defoliation.
  • Li Gan, Gao Lili, Kang Zhenghua, Li Jianwei, Wang Mifeng, Ma Yunzhen, Zhang Jusong
    Cotton Science. 2016, 28(4): 352-360. https://doi.org/10.11963/issn.1002-7807.201604006
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    The aim of this study was to examine the regularity of nutrient migration in machine-harvested cotton under different water treatments under natural conditions in northern Xinjiang and to provide a theoretical basis for the efficient use of water and nitrogen to promote the cultivation of machine-harvested cotton. The machine-harvested variety Xinluzao 57 was used as the study material. Three different irrigation frequencies 10 (D10), 8 (D8), and 6 (D6), with a drip irrigation quota of 4500 m3·hm-2 were established. Results showed that under this drip irrigation quota, the plant-type structure of treatment D8 was most suitable, because its accumulations of dry matter and nitrogen were appropriate, and the proportions of dry matter accumulation and nitrogen distributions of each organ were suitable. In addition, the single boll weight and boll number per plant of cotton increased, so it can increase the cotton yield. Therefore, the water allocation of the D8 frequency, combined with a corresponding irrigation system, can achieve a high yield of cotton.
  • Ren Fengxiao, Sun Hongchun, Zhang Yongjiang, Bai Zhiying, Liu Liantao, Zhang Ying, Chen Jing, Li Cundong
    Cotton Science. 2016, 28(4): 361-368. https://doi.org/10.11963/issn.1002-7807.201604007
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    To consider the difference of cotton yield and the field micro-environment under different canopy structures in the Yellow River basin, we studied cotton strains Jimian 958 and Nongdamian 601 under four different canopy structures. Different canopy structures were created through vegetative branch removal (T1, regular group), high (tow rows) and low (tow rows) plant-alternated vegetative branch removal (T2, irregular group), vegetative branch retention (T3, regular group), and high (tow rows) and low (tow rows) plant-alternated vegetative branch retention (T4, irregular group). The results showed that the light transmittances at the bottom of canopy in vegetable branch retention groups were significantly reduced in Jimian 958 and Nongdamian 601; however, the light transmittance at the bottom of the T4 group was significantly higher than that of the T3 group. The CO2 concentration has significant partial correlations with temperature and humidity in each stage. During the flowering and blooming stage, compared with CO2 concentration in the air, the CO2 concentration in the down layer of high and low plant-alternated group on average increased 8.69%―11.63%, and that in the middle layer reduced by 1.66%―4.24%. The within-canopy temperature decreased significantly in the T4 group, and the cotton group down-layer humidity and CO2 concentration increased significantly. The vegetative branch removal groups have a low abscission rate, and their seed cotton yields were significantly higher than those of the vegetation-retention groups. The seed cotton yields of the high and low plant-alternated groups were higher than the regular groups, especially the T4 groups of different varieties (Jimian 958 and Nongdamian 601) in which seed cotton yields increased by 15.79%, 10.81% higher than in the T3 group, respectively. Therefore, the vegetative branch retention group combined with high and low plant-alternated planting (T4) can improve the cotton lower-middle-class microclimate, reduce the abscission rate, and increase the number of bolls per unit area, which is helpful to increase production.
  • Wu Cuicui, Li Pengbo, Cao Meilian, Liu Huimin, Cao Cairong, Yang Liuliu
    Cotton Science. 2016, 28(4): 369-374. https://doi.org/10.11963/issn.1002-7807.201604008
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    Wall bees (Osmia) were used as pollinators on cotton cytoplasmic male sterile (CMS) lines in a net house for two years, with honeybee pollination as a control, to screen appropriate bee species for hybrid production. We observed the biological characteristics of wall bees and honeybees, and analyzed the traits of the cotton pollinated by those two kinds of bee  in the fields. The results showed that the adaptability of wall bees was better than that of honeybees, and weather factors had little effect on pollination. The activity of wall bees decreased substantially, when the temperature was greater than 35 ℃. In terms of single flower residence time, wall bees were extremely significantly longer than honeybee on cotton CMS and CMS maintainer. There were extremely significant differences in number of bolls per plant, number of seeds per boll, lint percentage between wall bees and honeybees pollination and the number of fruit branches per plant, boll weight also showed significant differences. Honeybee pollination provided the higher seed yield, but the cost of wall bee pollination was lower. We can take some measures such as improving field management and releasing wall bees for several times at the proper times,  to utilize the wall bees better in the pollination of cotton CMS.
  • Liu Ruixian, Zhang Guowei, Yang Changqin, Zhang Lei, Ni Wanchao
    Cotton Science. 2016, 28(4): 375-383. https://doi.org/10.11963/issn.1002-7807.201604009
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    To study effects of wheat straw on cotton(cv. CCRI 50) seed germination and root growth, we carried out an experiment with different concentrations of water extract and decomposition liquid of wheat straws in the environment chamber of Jiangsu Academy of Agricultural Sciences at Nanjing. Results showed that, contents of nitrogen, phosphorus, potassium and total phenolic acids increased with the increasing water extract and decomposition liquid concentrations (mass fraction). With the same concentration, there were no obvious difference about nitrogen and phosphorus contents between water extract and decomposition liquid, but total phenolic acids and potassium contents in decomposition liquid were higher than that in water extract. The water extract and decomposition liquid, not exceeding 7.0% (mass fraction) and 5.0%, individually, only delayed the germination time of cotton seed, but had no effects on germination rate. However, over 7.0% and 5.0% individually, they led to not only a delay of germination time, but also a decrease in germination rate. The water extract and decomposition liquid, over 3.0%, inhibited root elongation and lateral root development, which appeared as decrease of the lateral root number, total root surface area, and total root volume, but increase of average root diameter. The reduction of lateral root mainly resulted from short length of branched root zone. The water extract and decomposition liquid played a positive role at lower concentrations(below 3.0%), but an in hibitory role at higher concentrations (over 3.0%) on root respiration and root vigor; however, they had opposite effects on the content of malondialdehyde. Allelopathic effects of the extract and decomposition liquid were related to the total phenolic acids, nitrogen, phosphorus and potassium contents, which could inhibit seed germination, root morphology formation and root respiration. The allelopathic effects of decomposition liquid were higher than that of water extract.
  • Zhang Beibei, Geng Wei, Cui Jianyu, Mu Kangguo, Ma Ying, Hu Lin
    Cotton Science. 2016, 28(4): 384-391. https://doi.org/10.11963/issn.1002-7807.201604010
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    China produces an abundance of cotton byproducts each year, such as cotton straw and cotton seed. Cotton byproducts make useful biomass energy resources as there is high cellulose and lignin content in cotton straw and cotton seed shells, and high oil content in cotton seed kernels. From 1980 to 2014, the main cotton-producing regions in China expanded to Xinjiang in the northwest region, while the cotton planting area in the Yellow River and Yangtze River regions decreased. Cotton byproducts for bioenergy production could provide a new way to relieve energy pressure in China, and enhance the benefits associated with cotton production. Using cotton production data from 1980 to 2013 covering all cotton-producing provinces in China, we analyzed regional distributions and changes in the main cotton-producing regions over the past three decades to determine production and energy potentials of cotton byproducts. In China, the theoretical production of cotton byproducts in 2014 was estimated at 32.44 million tons (Mt); comprised of 22.36 Mt cotton straw and 10.08 Mt cotton seed. Together, cotton straw and cotton seed shells could produce 10.84 Mt biochar or 13.55 Mt bio-oil, and cotton seed kernel could produce 1.52 Mt bio-diesel. The total energy potential of cotton byproducts in China in 2014 reached 10.00 Mt standard coal equivalence. If 30% or 50% of cotton byproducts can be converted into biomass energy, the energy potential would be 3.00 Mt or 5.00 Mt standard coal equivalence, respectively. The cotton byproducts for bioenergy and its industrial development will not only be helpful to relieve pressure on the energy supply, but also contribute to sustainable development of the cotton industry and increase the income of cotton farmers.
  • Feng Xiaoxu, Zou Changsong, Lu Cairui, Cheng Hailiang, Zhang Youping, Mkulama A P Mtawa, Wang Qiaolian, Song Guoli
    Cotton Science. 2016, 28(4): 392-398. https://doi.org/10.11963/issn.1002-7807.201604011
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    The genetic analysis and cloning of genes associated with cotton fiber characteristics will not only reveal the mechanism of fiber development, but will also aid molecular breeding. The cotton (Gossypium arboretum) mutant DPL972 is fuzzless, providing a crucial germplasm resource for cotton research. An F2 population derived from a cross between the wild-type DPL971 and the mutant DPL972 was constructed, and the results of trait investigation fitted a segregation ratio of 3∶1, which indicated that the fuzzless trait was controlled by a single dominant gene (named here as GaFzl). A linkage map was constructed, which included 15 simple sequence repeat markers close to the candidate target gene. The fuzzless gene GaFzl was inferred to be located on chromosome A08 and was tightly linked with marker SSR82 at a genetic interval of 6.6 cM.
  • Wang Zhenyu, Li Wei, Zhou Xiaojian, Pei Xiaoyu, Liu Yangai, Zhou Kehai, Zhang Wensheng, Meng Qingqin, Wang Haifeng, Ge Yong, Li Ying, Liu Junfang, Ma Xiongfeng, Yang Daigang
    Cotton Science. 2016, 28(4): 399-406. https://doi.org/10.11963/issn.1002-7807.201604012
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    Single nucleotide polymorphism (SNP) markers have been widely used in crop studies and significant progress has been made in recent years. In this article, we examine the development and research progress of SNP markers in cotton, including the methods of using gene chips, simplifying genome sequencing and resequencing to develop the SNP markers in cotton, and the applications of SNP markers in cotton such as genetic mapping, quantitative trait loci analysis, molecular marker-assisted breeding, genome sequencing, and phylogenetic analysis. The present problems in the development and application of SNP markers on homologous and homoeologous loci in allotetraploid cotton are also discussed. The anticipated prospects for SNP markers are also discussed in terms of rapid detection and quantitative trait loci mapping, which is expected to help the development and application of SNP markers in cotton in future.
  • Hu Wenran, Fan Ling, Sun Tao, Xie Lixia, Tian Xiaoli
    Cotton Science. 2016, 28(4): 407-412. https://doi.org/10.11963/issn.1002-7807.201604013
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    In the plant cell wall, the phenylpropane monomer affects the properties of the polymers derived from phenylpropanoid compounds. Recent studies have found that the phenylpropane metabolism (the second largest metabolic pathway after the glucose metabolism) exists in developing cotton fibers. Phenylpropanoid compounds present in cotton fiber are related to the formation of quality fiber. Because the phenylpropanoid compounds contents of cotton fibers are very low, and mature cotton fibers have dead, hollow, soft, dried cell walls that are difficult to triturate with conventional grinding techniques, the existing methods used to directly analyze the phenylpropanes monomer are unsuitable for use in cotton fibers. In this experiment, to evaluate phenylpropanoid monomers in cotton fibers, phenylpropanoid compounds were extracted from cotton fiber using thioglycolic acid, then using the derivatization followed by the reductive cleavage method and gas chromatograph analysis, the phenylpropanoid monomers of cotton fiber were determined. The results showed that guaiacyl monomer (G-lignin) and syringyl monomer (S-lignin) are present in cotton fibers. This study will lay a basis for further analysis of phenylpropanoid compounds in cotton fiber.
  • Zhang Youchang, Feng Changhui, Bie Shu, Wang Xiaojiao, Yi Xianda, Zhang Cheng, Qin Hongde
    Cotton Science. 2016, 28(4): 413-417. https://doi.org/10.11963/issn.1002-7807.201604014
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    An improved method of rapid simple DNA extraction from cotton leaves was established based on the TPS (named by using TPS buffer—a mixture of Tris-HCl, EDTA and KCl) procedure to increase the efficiency of molecular marker-assisted selection. Compared with cetyltrimethyl ammonium bromide (CTAB) and TPS procedures, this new method is superior as it uses fewer reagents, and is simpler, faster, and less expensive. This new method makes use of a pre-buffer with 2% (mass fraction) PVP40 added to the TPS buffer (0.1 mol·L-1 Tris-HCl, 0.01 mol·L-1 EDTA, 1.2 mol·L-1 KCl), a 96-well PCR plate, and a miniature hand drill to grind samples. This new method could omit protein extraction, DNA precipitation, washing, drying, and dissolving procedures, and does not require chloroform or isoamyl alcohol. Using this method, more than 600 DNA samples can be extracted in a day by one person, and the extracted DNA is suitable for SSR (simple sequence repeat) analysis. Thus, the method can be used in cotton molecule marker-assisted selection.
  • Ma Yanbin, Sun Xuan, Wu Xia, Lin Chaoyang, Liu Chengyi, Wang Xia, Zhang Shuwei, Wang Xinsheng, Zhang Linshui, Shen Zhicheng, Li Yan'e
    Cotton Science. 2016, 28(4): 418-424. https://doi.org/10.11963/issn.1002-7807.201604015
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    It is important to cultivate transgenic cotton varieties with glyphosate-resistance to enhance cotton production in China. This research aimed to obtain transgenic cotton with a new glyphosate-resistant gene that was initially obtained by Zhejiang University. All of the 65 independent transgenic events of the regeneration cotton plants were obtained through the tissue culture technology using a glyphosate-resistant screen. For these transgenic regeneration plants, improving the transgenic efficiency of the regenerated plant is very important in transgenic research. First, a 1.7-kb fragment of exogenous gene of all 65 regeneration plants was detected respectively through a special primer of G10evo-EPSPS with PCR amplification. The result showed that the rate of positive transgenic plants could reach the high level of 66.7%―100%, with the average value of 81.5%. We then used these fragment characters to verify the positive segregating progenies of transgenic cotton with PCR amplification. In addition, protein antibodies were used in the western-blot experiment for selecting the resistant plants, in which the 46-kDa hybridization bands were tested. The statistical result of the test showed that the positive transgenic plants rate reached 55.4%. Based on the positive lines verifiedby PCR and western-blot, the generations of transgenic cotton were selected by spraying glyphosate herbicides onto the test field. On this basis, two transgenic lines with excellent resistance were confirmed, both of which have a high utilization value for the yield and resistance performance of cotton breeding materials in the future.