15 September 2015, Volume 27 Issue 5
    

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  • Jin Xin, Chang Jing, Si Huaijun, Zhang Ning, Wu Jiahe
    Cotton Science. 2015, 27(5): 385-390. https://doi.org/10.11963/issn.1002-7807.201505001
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    The biosafety of transgenic plants can largely affect their planting area, speed of spread, and acceptance and sale in the market for transgenic products. In this study, we employed virus-induced gene silencing (VIGS) technology to inhibit nptⅡ transcript expression in transgenic Bt cotton. The pYL-156-nptⅡ construct was generated by inserting an nptⅡ gene fragment into the tobacco rattle virus(TRV) vector pYL-156. The plants were transformed with Agrobacterium harboring pYL-156-nptⅡ by injecting cotyledons with a 1-mL no-needle syringe. Two weeks later, 0.5% (mass concentration) kanamycin was applied to the leaves of the silenced plants. The leaves of the silenced plants treated with kanamycin showed necrotic spots, but control leaves grew normally. Based on RT-PCR and qPCR analyses, the transcript level of nptⅡ in the leaf, root and stem in the silenced plants was reduced by 99.9%, 92.5% and 98.5%, respectively, compared with control plants; similar nptⅡ transcript levels were observed in silenced plant leaves at 30 and 60 days after injection. Our results demonstrate that foreign genes in transgenic plants, especially selectable marker genes, can be eliminated or inhibited by post-transcriptional gene silencing, which could help to reduce the accidental escape and unpredicted risks of transgenic plants growing in an open environment.
  • Xu Zhenzhen, Guo Qi, Liu Jing, Xu Peng, Zhang Xianggui, Shen Xinlian
    Cotton Science. 2015, 27(5): 391-400. https://doi.org/10.11963/issn.1002-7807.201505002
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    DnaJ proteins comprise a large family and have been shown to be involved in various stress responses in recent studies. In this study, we systematically studied the number, types, chromosome distribution, subcellular localization and evolutionary relationships of DnaJ proteins in the Gossypium raimondii using a bioinformatics method. In parallel, we studied the expression patterns of DnaJ proteins in G. aridum and G. klotzschianum under salt stress. 119 DnaJ family members were identified with an uneven chromosome distribution and various subcellular localizations. The amino acid lengths of the DnaJ protein family members varied from 73 to 2574. Their DnaJ domains could be divided into nine groups, and there were homologs of Arabidopsis thaliana genes in each group. Three DnaJ gene family members were differentially expressed under salt stress in both G. aridum and G. klotzschianum, and two genes were differentially expressed at every time point under salt stress in G. aridum. The identification and bioinformatics analysis of the DnaJ gene family in G. raimondii will provide a firm foundation for future study of these genes' functions in cotton.
  • Geng Jibiao, Zhang Min, Li Chengliang, Zheng Wenkui, Tian Xiaofei
    Cotton Science. 2015, 27(5): 401-407. https://doi.org/10.11963/issn.1002-7807.201505003
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    A two-year pot experiment was conducted to compare the effects of two controlled-release nitrogen fertilizers (CRNF) including polymer and sulfur-coated urea (PSCU) and polymer-coated urea (PCU), common urea (U) and no nitrogen (N) treatments with the same amounts of phosphorus (P) and potassium (K) on the yield of cotton and changes of soil nutrients. The results indicated that the cotton yield in CRNF treatments was significantly increased by 6.78%-28.91% compared with the basal urea application treatment, but there was no significant difference between the CRNF treatments and the treatment with split urea fertilization at pre-sowing (40%) and the first bloom stage (60%). The boll weight was increased by 2.5%-11.32% with CRNF compared with the basal urea application treatment. However, the type of N fertilizer had no effect on the lint percentage, which remained at 44.14%-46.67%, or boll numbers. The soil NO3-N and NH4+-N contents were increased in the CRNF treatments from the peak bloom stage to the initial boll-opening stage compared with the basal urea application treatment. The soil NO3-N and NH4+-N contents were increased by 20.98%-79.25% and 12.51%-29.12%, respectively, in the peak bloom stage, and 50.31%- 171.24% and 21.87%-40.57% in the initial boll-opening stage. At the same time, the available K content in the soil was increased with CRNF from the first bloom stage to the peak bloom stage compared with U treatment. Thus, one-time CRNF application not only met the demand for N over the whole growth period of potted cotton, but also preserved the yield and decreased the labor cost of fertilizer dressing.
  • Chen Jing, Liu Liantao, Wang Yafei, Sun Hongchun, Zhang Yongjiang, Li Cundong, Lu Zhanyuan
    Cotton Science. 2015, 27(5): 408-416. https://doi.org/10.11963/issn.1002-7807.201505004
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    The objective of our study was to investigate the effects of N-fertilization rate on the morphological and physiological characteristics of the "boll-leaf system" in the upper fruit branches of cotton. To achieve this, we conducted a two-year experiment with Lumianyan 28 as a material. There were four levels of nitrogen fertilization: control treatment (N0, 0 kg·hm-2), low nitrogen (N1, 120 kg·hm-2), moderate nitrogen (N2, 240 kg·hm-2) and high nitrogen (N3, 480 kg·hm-2). The main results were as follows: 1) nitrogen fertilization improved the branch length and leaf area, and decreased the diameter ratio of near and far branches. The differences between the control treatment and the moderate and high treatments were significant. 2) Chlorophyll and soluble protein contents in the upper fruit branches' leaves and seeds were increased by nitrogen fertilization. The chlorophyll and soluble protein contents in the upper fruit branches' leaves in the moderate treatment were significantly higher than in the control. The soluble protein content in the upper fruit branches' seeds in the moderate treatment was significantly higher than in the control. 3) With increasing nitrogen fertilizer, the boll weight of the upper fruit branches first increased and then decreased. The boll weight in the moderate treatment was higher than in the other treatments. At the late bolling stage, the differences between the moderate and control treatments became significant. The fiber quality was increased by N application, and the moderate treatment had the best quality in the upper fruit branches. The morphological characteristics, physiological levels and fiber qualities were significantly affected by the N rate. "Source-sink" coordination in the upper fruit branches could be enhanced by the proper amount of N. In this study, moderate nitrogen(240 kg·hm-2) coordinated the growth of the "boll-leaf system" of the upper fruit branches the best.
  • Rong Fuxi, Tang Likui, Tang Yuanyuan, Li Zhibo, Zhao Ruihai, Lei Jun, Wei Yinong
    Cotton Science. 2015, 27(5): 417-426. https://doi.org/10.11963/issn.1002-7807.201505005
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    In this study, a genetic linkage map was constructed for an F2 population of 195 cotton plants derived from the cross between the parental introgression lines 13-1 and Liao 12 with SSR(simple sequence repeats) markers using the JoinMap 3.0 software. The map included 39 SSR loci and 13 linkage groups, covering 1174.4 cM and accounting for 26.7% of the cotton genome. Based on the inclusive composite interval mapping method with IciMapping, 44 QTLs(quantitative trait locus) were identified on eight chromosomes in the F2:3 population, including 30 QTLs for chlorophyll fluorescence parameters, seven for leaf dry material content, six for leaf area index, and one for chlorophyll content. Multiple trait QTLs were located between the common range of markers on the same chromosome and the additive effect of some QTLs were inherited from the same parent. Some QTLs related to dry material content and maximal photochemical efficiency of PSⅡ appeared repeatedly in different ranges on three chromosomes and QTLs associated with leaf area index and maximal photochemical efficiency of PSⅡ recurred in different ranges on four chromosomes. These findings show that pleiotropism or genetic linkage could be used to combine many high-photosynthesis-efficiency genes into plants by genetic engineering.
  • Cotton Science. 2015, 27(5): 427-434. https://doi.org/10.11963/issn.1002-7807.201505006
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    Under the ecological and climatic conditions of Xinjiang, differences in the temporal and spatial distribution characteristics of the roots, aboveground biomass, and root/shoot ratio in cotton fields of different yield levels were studied using the soil profile sampling method and Xinluzao 43 and 45 as experimental materials. The results showed that the root biomass, proportion of roots in the middle and lower soil layers, total biomass, and aboveground biomass in super high-yield fields was significantly increased throughout the growth season compared with common high-yield and low-yield fields. However, the root/shoot ratio was decreased. The yield components exhibited higher total boll numbers and weight. Under super high-yield conditions, the large root biomass and total biomass were mainly attributable to earlier initial time and higher rate of the rapid biomass accumulation, than under the other yield levels. This suggested that the yield of cotton varieties could be increased according to their growth characteristics, by appropriate early sowing in advance of normal growth of vegetative organs and an appropriate delay without water or water transfer measures, to ensure water supply to reproductive organs at the late growth stage and increase the boll weight by increasing the proportion of roots in the middle and lower soil layers, decreasing the root/shoot ratio, and increasing assimilate transfer from leaves to bolls.
  • Su Zhifeng, Yang Wenping, Yang Zhenping, Du Tianqing, Hao Jiaomin, Sun Min, Gao Zhiqiang
    Cotton Science. 2015, 27(5): 435-444. https://doi.org/10.11963/issn.1002-7807.201505007
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    Immature loess subsoil was used to find the optimum fertilization regime to improve the productivity and vertical distribution of root dry-matter weight of cotton (Gossypium hirsutum) by soil column culture in root-tubes for two years. Four kinds of fertilizer (N, P, NPK, and organic fertilizer) and fertilization of three soil layers (at depths of 0-20 cm, 40-60 cm and 80-100 cm) were used to investigate the dry-matter weights of the stem-leaf and boll, and the vertical distribution of root dry-matter weight in cotton. The control treatment (CK) was without fertilization. The highest dry-matter weights for the stem-leaf and boll of cotton were observed under organic fertilizer at a 40-60 cm soil depth, followed by NPK fertilizer at a 40-60 cm soil depth, and there was a significant difference between them (P<0.05). A higher root dry-matter weight in cotton was observed under P or NPK fertilizer at a 40-60 cm soil depth than under the other treatments, and the root dry-matter weight percentage below the 40 cm root layer was higher than in the other root layers. Among all treatments, the root dry-matter weight percentage in the 0-20 cm root layer was the highest, representing 40%-80% of the total (0-100 cm). Overall, using organic fertilizer at a 40-60 cm soil depth is the optimum fertilization method  in current year for planting cotton in immature loess subsoil fields.
  • Li Shutian, Xing Suli, Cui Rongzong, Zhang Yan
    Cotton Science. 2015, 27(5): 445-453. https://doi.org/10.11963/issn.1002-7807.201505008
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    To determine the soil potassium status and supply capacity as well as their relation to cotton yield and quality in major cotton areas in north China, surface soil (0-20 cm), cotton plant and lint samples were collected and analyzed from representative cotton fields in Pingyuan County of Shandong Province, Weixian County of Hebei Province and Changji City of Xinjiang Province in 2012 and 2013. In Pingyuan County, more than 50% of the soil samples had 100-200 mg· kg-1 soil available K and 23% of samples had more than 300 mg·kg-1, with an average of 217 mg·kg-1. In Weixian County, 53% of soil samples had 100-150 mg·kg-1 soil available K and 22% of samples had less than 100 mg·kg-1, with a mean of 128 mg·kg-1. In Changji, more than 2/3 of samples had soil available K of 150-300 mg·kg-1 and 25% of samples had more than 300 mg·kg-1, with an average of 232 mg·kg-1. The soil K supply capacity, represented by uptake K of aboveground organs, was 120-140 kg·hm-2 in 68% of sites with a mean of 128.2 kg·hm-2 in Pingyuan County, 80-120 kg·hm-2 in 65% of sites with a mean of 105.1 kg·hm-2 in Weixian County, and 150-300 kg·hm-2 in 92% of sites with an average of 213.6 kg·hm-2 in Changji City. The average K uptake by aboveground organs required to produce 100 kg of lint cotton was 9.77, 7.82 and 12.25 kg, respectively, in Pingyuan County, Weixian County and Changji City. In Pingyuan County, significant positive correlations existed between soil available K and lint cotton yield, plant K uptake when the soil available K was less than 123 mg·kg-1. In Weixian County, there were no significant correlations between soil available K and lint cotton yield, plant K uptake. In Changji, significant positive correlations existed between soil available K and lint cotton yield, plant K uptake when the soil available K was less than 220 mg·kg-1. The correlations between plant K uptake and cotton yield varied by location, but there were significant positive correlations between lint K uptake and cotton yield in all three sites. There were great variations in the relationships between plant K uptake, lint K uptake, lint K content and fiber quality indices in different locations.
  • Lu Yanyan, Zhang Min, Tian Xiaofei, Geng Jibiao, Li Chengliang, Ma Jinzhao, Yang Jie
    Cotton Science. 2015, 27(5): 454-462. https://doi.org/10.11963/issn.1002-7807.201505009
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    To explore the effects of copper-based protective agents on the growth, yield, and disease control efficiency of cotton, pot experiments were conducted in 2013 and 2014. The results showed that the lint yield was increased by 4.22%-13.38% and 21.89%-38.36% by spraying four kinds of copper-based protective agents on leaves with the concentration of 1 g·L-1 and 2 g·L-1, respectively, compared with water. The aboveground dry matter weight of cotton plants was improved by 7.44%-19.38% and 10.25%-23.96%, compared with spraying water on the leaves. Additionally, the disease index was reduced by 26.75%-40.91% and 22.78%-37.78% by spraying copper-based protective agents on the leaves, compared with water. There was no difference among the different kinds of treatments. Based on these analyses, we conclude that the application of different concentrations and kinds of copper-based protective agents can promote cotton growth and production. Also, the copper-based protective agents had favorable disease control efficiency for cotton Verticillium wilt. Thus, these copper-based protective agents have good application value.
  • Liu Tao, Tao Rui, Li Jun, Chu Guixin, Liang Yongchao, Lei Jun
    Cotton Science. 2015, 27(5): 463-468. https://doi.org/10.11963/issn.1002-7807.201505010
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    A field experiment was carried out to study the effect of nitrapyrin on biomass, nitrogen uptake and nitrogen use efficiency (NUE) in cotton plants under drip irrigation conditions using a one-factor randomized block in 2012 and 2013. Three treatments were given as follows: CK (no nitrogen input), urea (225 kg·hm-2 nitrogen input) and urea + nitrapyrin (225 kg·hm-2 nitrogen and 2.25 kg·hm-2 nitrapyrin input). The results in both years showed that the biomass, nitrogen uptake and yield of cotton plants were increased when urea and nitrapyrin were applied under irrigation conditions. In the urea + nitrapyrin treatment, the biomass and nitrogen uptake of aboveground cotton organs increased by 4.1%-5.1% and 4.3%-4.4%, respectively, and the lint yield increased by 4.1%-4.4% compared with the urea treatment. The biomass of the stems, leaves, and buds-flowers-bolls increased by 2.7%-4.5%, 14.9%-16.2% and 2.5%-3.9%, and the nitrogen uptake increased by 0.4%-1.1%, 12.2%-16.3% and 2.9%-3.4%, respectively. The NUE was significantly improved by 11.5%-12.5% after nitrapyrin was added. The results will provide theoretical basis for high nitrogen fertilizer utilization in drip-irrigated cropland by application of nitrapyrin.
  • Zhang Jingxia, Wang Furong, Gao Yang, Zhang Jun
    Cotton Science. 2015, 27(5): 469-473. https://doi.org/10.11963/issn.1002-7807.201505011
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    Virus-induced gene silencing(VIGS) is a kind of post-transcriptional gene silencing that is common in plants and has been widely applied to study gene functions as a reverse genetics technique because of its rapidity, high efficiency and throughput characteristics. In recent years, VIGS has been used in the research of gene functions in cotton. This paper reviews the principles of the VIGS technology system in cotton, especially summarizing the applications of VIGS in the identification and functional research of genes related to disease resistance, fiber quality, and development. Finally, the applications of VIGS in cotton research are discussed.
  • Wang Xinling, Ma Xiaoyan, Jiang Weili, Ren Xiangliang, Ma Yajie, Ma Yan
    Cotton Science. 2015, 27(5): 474-480. https://doi.org/10.11963/issn.1002-7807.201505012
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    Recent developments and the current status of research on the competition between cotton and weeds are summarized, including the influence of weeds on cotton production, growth, quality and farming operations. The main measures used to reflect the competitive relationship between weeds and cotton include the species composition, density and competition period, and researches have focused on analyzing the final yield and growth indicators of cotton. However, studies on the inherent mechanism of competition between cotton and weeds are scarce. Thus, it is essential to clarify the morphological changes in cotton and explain the physiological and ecological mechanisms of the cotton response to weed stress on both the external environmental and internal physiological levels, and then further explore the variation mechanism of competition between cotton and weeds.
  • Yang Xiuyi, Tian Xiaofei, Zhang Juan, Geng Jibiao, Zhang Min, Li Chengliang
    Cotton Science. 2015, 27(5): 481-488. https://doi.org/10.11963/issn.1002-7807.201505013
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    Nitrogen plays a vital role in cotton growth and metabolism. Controlled release nitrogen fertilizer(CRNF) can control the supply of nitrogen based on the requirements of cotton, thus improving the fiber quality and yield, and increasing the nitrogen use efficiency. The application of CRNF has sped up the development of simplified cotton cultivation technology with high yield and efficiency, and also decreases environmental pollution. The concept and types of CRNF are described and research advances in the effects of CRNF on yield and its components, fiber quality, the physiological characteristics of cotton and soil nitrogen dynamics are reviewed in the present paper. Additionally, the problems, research direction and outlook of CRNF for cotton applications are discussed.
  • Cotton Science. 2015, 27(5): 489-493. https://doi.org/10.11963/issn.1002-7807.201505014
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    The effects of an exogenous EPSPS gene on the agronomic traits and fiber quality of upland cotton were studied, using the transgenic glyphosate-resistant cotton germplasm lines G6-1 to G6-19 as materials and their non-transgenic genetic background cultivar, CCRI 49, as a control. The results showed that integration of the exogenous gene into cotton had a major impact on fiber quality factors, for instance fiber upper half mean length, micronaire, breaking elongation, and breaking tenacity, and also on agronomic traits such as lint and boll weight. The variation range of lint was 30.5%-42.7%, and lint variation was heritable according to the correlation analysis of T1 and T2. The maximum and minimum values of boll weight were 6.5 g and 4.6 g, respectively. These results suggested that the breeding of transgenic cotton should focus not only on goal-oriented characters but also non-target traits during selection.