Verticillium Wilt Resistance of A Transgenic Cotton Line with Chitinase and Glucanase Genes
2011, 23 (1):
Artificial disease nursery, severe disease field and greenhouse were jointly used for Verticillium wilt disease resistance identification of a transgenic cotton line 61217-1 into which chitinase and glucanase genes were transferred. Results showed that transgenic line 61217-1 exhibited high resistant level to Verticillium wilt disease in artificial nursery during successive three years. Average disease index of line 61217-1 was 17.9, which was significantly lower than that of the acceptor CCRI 24. In 2009, in the severe disease fields, the average disease indexes of this transgenic line were 18.7, 18.1 and 16.7 in Anyang, Henan Province, Dafeng, Jiangsu Province, and Shihezi, Xinjiang, respectively. These indexes were obviously lower than those of the acceptor CCRI 24, respectively. Furthermore, this chitinase-glucanase transgenic cotton line 61217-1 also showed higher resistant to strong virulent defoliating Verticillium dahliae, which was isolated from Hebei, Hubei and Xinjiang, respectively. And their average disease indexes were 16.4, 16.8 and 15.6, which were significantly lower than that of CCRI 24, respectively. The results indicated that the transgenic cotton line 61217-1 (Chi+Glu) exhibited excellent and stable resistance to Verticillium wilt disease.
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