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Cotton Science ›› 2020, Vol. 32 ›› Issue (5): 381-391.doi: 10.11963/1002-7807.ljlb.20200729

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Effect of Coronatine on AsA-GSH Cycle of Cotton Seedling under Low Temperature Stress

Li Jin1, Zhai Menghua2, Yu Chunxin3, Wang Li2, Zhang Jungao1, Zhou Xiaoyun1, Liang Jing1, Duan Liusheng3*,Lei Bin1*   

  1. 1. Institute of Nuclear Technology and Biotechnology, Xinjiang Academy of Agricultural Sciences/Key Laboratory of Crop Ecophysiology and Farming System in Desert Oasis Region, Ministry of Agriculture, Urumqi 830091, China; 2. College of Agriculture, Xinjiang Agricultural University, Urumqi 830052, China; 3. College of Agronomy and Biotechnology, China Agricultural University/Engineering Research Center of Plant Growth Regulator, Ministry of Education, Beijing 100193, China
  • Received:2020-03-27 Online:2020-09-15 Published:2020-09-29

Abstract: [Objective] In order to explore the stress-resistant mechanism of coronatine, an indoor experiment was conducted to study the effect of coronatine on ascorbate-glutathione circulatory system of the vegetative organs of cotton seedling under low temperature stress. [Method] Xinluzao 57 was used as the experimental material, when the seedlings reached the two-leaf stage, they were treated at 25 ℃ and 4 ℃ after spraying water, marked as CK and LT; the same treatment was conducted after spraying 0.01 μmol·L-1 coronatine, marked as COR and (LT+COR). After 1 d of treatment, the roots, stems and leaves of cotton seedling were collected to determine the antioxidant contents and antioxidant enzyme activities. [Result] Compared with the control treatment (CK), the APX, MDHAR activities and DHA, GSH, glutathione contents decreased, the GPX activity, AsA and ascorbate contents increased, and no significant effect on DHAR and GR activities were observed in roots, stems and leaves of cotton seedling after spraying water at low temperature (LT), while in leaves, the GSSG content decreased, AsA-DHA ratio and GSH-GSSG ratio showed the maximum increase. Compared with the LT treatment, spraying COR at low temperature (LT+COR) showed increased APX, MDHAR, DHAR, GPX, GR activities and AsA, DHA, GSH, ascorbate contents; glutathione content in roots, stems and leaves of cotton seedling, and GSSG content, AsA-DHA ratio and GSH-GSSG ratio obviously changed in leaves. [Conclusion] Spraying COR at low temperature could regulate the AsA-GSH metabolism and alleviate the damage caused by low temperature to cotton seedling. COR had the strongest relieving effect on seedling leaves.

Key words: cotton, low temperature stress, coronatine, AsA-GSH cycle, physiological characteristics