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棉花学报 ›› 2021, Vol. 33 ›› Issue (5): 393-403.doi: 10.11963/cs20210017

• 研究与进展 • 上一篇    下一篇

基于隶属函数法对不同基因型棉种萌发期抗氧化能力的综合评价

王洪博1(),付媛媛2,杨鸿基2,孙文君1,高阳3,*(),王兴鹏1,*()   

  1. 1.塔里木大学水利与建筑工程学院,新疆 阿拉尔 843300
    2.塔里木大学植物科学学院,新疆 阿拉尔 843300
    3.中国农业科学院农田灌溉研究所,河南 新乡 453000
  • 收稿日期:2021-03-02 出版日期:2021-09-15 发布日期:2022-01-27
  • 通讯作者: 高阳,王兴鹏 E-mail:18083915561@163.com;gaoyang@caas.cn;13999068354@163.com
  • 作者简介:王洪博(1995―),男,讲师, 18083915561@163.com
  • 基金资助:
    国家重点研发计划(2016YFC0400208);新疆生产建设兵团科技攻关项目(2018AB027);新疆维吾尔自治区研究生科研创新项目(XJ2019G265)

Comprehensive evaluation of antioxidant ability of different genotypes of cotton in germination period based on subordinate function method

Wang Hongbo1(),Fu Yuanyuan2,Yang Hongji2,Sun Wenjun1,Gao Yang3,*(),Wang Xingpeng1,*()   

  1. 1. College of Water Resource and Architecture Engineering, Tarim University, Aral, Xinjiang 843300, China
    2. College of Plant Science, Tarim Univercity, Aral, Xinjiang 843300, China
    3. Farmland Irrigation Research Institute, Chinese Academy of Agricultural Sciences, Xinxiang, Henan 453002, China
  • Received:2021-03-02 Online:2021-09-15 Published:2022-01-27
  • Contact: Gao Yang,Wang Xingpeng E-mail:18083915561@163.com;gaoyang@caas.cn;13999068354@163.com

摘要:

【目的】评估特早熟棉花品种中棉619的抗旱、耐盐、耐寒性以及逆境下的抗氧化能力。【方法】基于正交试验设计,用PEG模拟干旱胁迫、NaCl模拟土壤盐分胁迫、生化培养箱控制环境温度,以南疆膜下滴灌主栽品种新陆中37为对照,采用主成分分析、隶属函数法及聚类分析方法,对比分析了水、盐、温及其交互作用对两个棉花品种萌发期可溶性蛋白含量、淀粉酶及抗氧化保护酶活性等生理生化指标的影响。【结果】主成分分析表明:在水、盐、温及其交互作用下,超氧化物歧化酶活性和过氧化氢酶活性可以作为棉种萌发期抗旱、耐盐、耐寒性鉴定的生理生化指标;通过隶属函数法得出:15%(质量分数)PEG、0.4%(质量分数)NaCl及13 ℃条件下,中棉619抗氧化酶活性达到最高;新陆中37抗氧化酶活性在5% PEG、0.4% NaCl及19 ℃条件下达到最高。【结论】抗逆性强的品种一般具有较高的抗氧化能力,中棉619的抗旱及耐寒性高于新陆中37,对环境的适应能力较高,具有较高的抵抗环境胁迫的能力,可为棉花无膜种植栽培提供理论依据。

关键词: 棉种萌发; 环境胁迫; 隶属函数; 聚类分析; 抗氧化酶

Abstract:

[Objective] This study aims to evaluate the drought, salt, and cold tolerance and correspondence antioxidant enzymes activity of an extra early maturing cotton variety, Zhongmian 619. [Methods] An orthogonal experimental design was used to setup stress conditions, where as PEG was used to simulate drought stress, NaCl was used to simalate as salt stress, and temperature was modulated by the growth incubator. By the principal component analysis, subordinate function method and cluster analysis, the effect of water, salt and temperature on the physiological and biochemical indexes such as soluble protein content, the axtivity of amylase and antioxidant protective enzymes during the germination period of the Xinluzhong 37 and Zhongmian 619 were compared and analyzed. Xinluzhong 37, which is the main cultivar of under-film drip irrigation in South Xinjiang, was used as the control. [Results] Principal component analysis showed that the activity of superoxide dismutase (SOD) and catalase (CAT) could be used as physiological and biochemical indicators for the evaluation of drought, salt and cold resistance/tolerance of cotton seeds during the germination period. By the subordinate function method, the antioxidant enzymes activities of Zhongmian 619 were at the highest level in 15% PEG, 0.4% NaCl and 13 ℃ treatment, while those of Xinluzhong 37 were at the highest level in 5% PEG, 0.4% NaCl and 19 ℃. [Conclusion] Cultivars with stronger stress resistance generally have higher antioxidant capacity. Zhongmian 619 is more resistant to drought and cold than Xinluzhong 37, and has a higher ability to adapt to environmental stress, which can provide theoretical basis for cotton cultivation without film.

Key words: cotton seed germination; environmental stress; subordinate function method; cluster analysis; antioxidant enzymes