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棉花学报 ›› 2019, Vol. 31 ›› Issue (1): 12-22.doi: 10.11963/1002-7807.qzyfsl.20190115

• 研究与进展 • 上一篇    下一篇

棉花重组自交系铃重性状的QTL定位

曲朝阳1(),贾晓昀2,马启峰1,王寒涛1,魏恒玲1,范术丽1,*()   

  1. 1.中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南 安阳 455000
    2.河北省农林科学院粮油作物研究所,石家庄 050035
  • 收稿日期:2018-03-15 出版日期:2019-01-15 发布日期:2019-01-15
  • 通讯作者: *范术丽 E-mail:quzhaoyangcaas@163.com;fsl427@126.com
  • 作者简介:曲朝阳(1991―),男,硕士研究生, quzhaoyangcaas@163.com
  • 基金资助:
    国家自然科学基金(31701474);棉花生物学国家重点实验室自主课题(CB2017C05)

QTL Mapping of Boll Weight Trait Based on Recombinant Inbred Lines in Gossypium hirsutum L.

Qu Zhaoyang1(),Jia Xiaoyun2,Ma Qifeng1,Wang Hantao1,Wei Hengling1,Fan Shuli1,*()   

  1. 1. Institute of Cotton Research of Chinese Academy of Agricultural Sciences / State Key Laboratory of Cotton Biology, Anyang, Henan 455000, China
    2. Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Science, Shijiazhuang 050016, China
  • Received:2018-03-15 Online:2019-01-15 Published:2019-01-15
  • Contact: *Fan Shuli E-mail:quzhaoyangcaas@163.com;fsl427@126.com

摘要:

【目的】铃重是构成棉花产量的基本因子之一。本研究旨在定位铃重QTL(Quantitative trait loci),为分析铃重遗传组成提供参考。【方法】以中棉所36和海陆渐渗系G2005组配的137个RILs(Recombinant inbred lines)家系为作图群体,利用RAD-seq(Restriction-site associated DNA sequencing)技术及SSR(Simple sequence repeat)标记,构建遗传连锁图谱,并对5个环境下的铃重进行QTL分析。【结果】构建了包含26个连锁群、6 434个标记、总长为 4 071.98 cM、标记间平均距离为0.63 cM的遗传图谱。采用WinQTLCart 2.5软件的复合区间作图法进行QTL定位,共得到32个铃重QTL,分布于15条染色体,单个位点解释的表型变异率为4.46%~15.84%;qBW-A4-1、qBW-A4-2、qBW-A5-2、qBW-D9-1和qBW-D9-2能够在2个环境中检测到,解释5.07%~15.84%的表型变异率。【结论】本研究定位的主效QTL可用于分析铃重遗传机理。

关键词: 棉花; 重组自交系; 铃重; QTL

Abstract:

[Objective] The aim of this study was to explore the quantitative trait locus (QTL) related to the boll weight. [Method] A single seed descended population of 137 recombinant inbred lines (RILs) was developed from the cross of upland cotton (Gossypium hirsutum L.) CCRI 36 and G2005, an introgression inbred line introgressed from G. barbadense. Using restriction-site associated DNA sequencing (RAD-seq), a genetic linkage map composed of 6 434 makers, including 6 295 single nucleotide polymorphisms (SNP) and 139 simple sequence repeat (SSR) markers, was developed from the RILs population. [Result] This map spanned 4 071.98 cM with an average distance of 0.63 cM between adjacent markers. QTL mapping was performed by using boll weight data of five environments through WinQTLCart 2.5 software. Thirty-two QTL, with 4.46%-15.84% explained phenotypic variation related boll weight, were detected and found distributing on 15 chromosomes. qBW-A4-1, qBW-A4-2, qBW-A5-2, qBW-D9-1, and qBW-D9-2 were detected in two environments, which explained 5.07%-15.84% of the phenotypic variation. [Conclusion] Major QTLs detected in this study will provide an important reference for analysis of the genetic mechanism of boll weight.

Key words: cotton; recombinant inbred lines; boll weight; QTL