陆地棉色素腺体与不同棉酚旋光体含量之间的相关性研究

doi:10.11963/1002-7807.lhzsj.20170818

摘要/Abstract

摘要： 【目的】研究不同基因型棉花不同器官的色素腺体与棉酚旋光体含量之间的相关性。【方法】以6个陆地棉材料中棉所12、中棉所12（无）、珂字棉312、珂字棉312（无）、IF11和冀棉14为对象，精确测定主要器官的色素腺体密度和大小，并采用柱前衍生拆旋方法进行高效液相色谱分析，测定其相应的棉酚旋光体含量，然后对色素腺体和棉酚旋光体含量进行相关性分析。【结果】低酚棉全株无色素腺体，但在各器官中仍能检测到低于0.02%（质量分数）的少量游离棉酚存在。有酚棉各器官均具有典型的色素腺体，其中棉仁的色素腺体最密，苞叶最稀；色素腺体大小（直径）以铃壳最大，花瓣和棉仁最小。不同器官中，总棉酚含量以棉仁最高，苞叶最小；棉仁和花瓣的右旋棉酚含量大于左旋棉酚，而萼片、铃壳、上部叶、中部叶和下部叶的左旋棉酚含量大于右旋棉酚。有酚棉的色素腺体密度与大小之间呈极显著负相关；而总棉酚含量与不同棉酚旋光体含量之间，以及左右旋棉酚含量之间呈极显著正相关。色素腺体密度与总棉酚含量以及不同棉酚旋光体含量之间呈极显著的正相关，而色素腺体大小与右旋棉酚含量之间呈显著的负相关。【结论】棉仁与花瓣的总棉酚含量存在高度的相关性，因此，可以通过花瓣的棉酚含量来判断棉仁的棉酚含量。

关键词: 陆地棉; 色素腺体密度; 色素腺体大小; 左旋棉酚; 右旋棉酚; 相关性分析

Abstract: [Objective] The aim of this study was to examine the correlation between pigment glands and (-)/(+)-gossypol contents in different organs of different upland cotton genotypes. [Method] Using upland cotton materials CCRI 12, CCRI 12 (glandless), Coker 312, Coker 312 (glandless), IF11 and Jimian 14, we accurately measured the density and size of the pigment glands, tested (-)/(+)-gossypol contents by pre-column derivatization-high performance liquid chromatography (HPLC), and then analyzed the correlation between pigment glands and (-)/(+)-gossypol contents. [Result] The whole plants of low gossypol cotton lines were without pigment glands, but free gossypol could still be detected in different organs at mass fractions less than 0.02%. The organs of glanded cotton all had typical pigment glands, the gland density was highest in the seed and lowest in the bract. The gland size (diameter) was largest in the boll shell and smallest in the petal and seed. Across different organs, the total gossypol content was highest in the seed and lowest in the bract. The contents of (+)-gossypol in the seed and petal were higher than (-)- gossypol. Conversely, the (-)-gossypol contents of the calyx, boll shell, upper leaf, middle leaf, and lower leaf were higher than (+)-gossypol. There was a significant negative correlation between the density and size of glands in glanded cotton. There were significant positive correlations between total gossypol content and (-)/(+)-gossypol contents, and between (+)-gossypol content and (-)-gossypol content. Gland density was positively correlated with total gossypol content and (-)/(+)-gossypol contents. Gland size was negatively correlated with (+)-gossypol content, and showed no significant correlation with (-)-gossypol content or total gossypol content. [Conclusion] Overall, the strong positive correlation between total gossypol content in the seed and petal suggests that we can use the petal gossypol content to infer the seed gossypol content.

Key words: upland cotton; pigment gland density; pigment gland size; (-)-gossypol; (+)-gossypol; correlation analysis

中图分类号:

S562. 01

刘慧, 季灵艳, 赵天伦, 陈进红, 祝水金. 陆地棉色素腺体与不同棉酚旋光体含量之间的相关性研究[J]. 棉花学报, 2017, 29(5): 437-446.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://journal.cricaas.com.cn/Jweb_mhxb/CN/10.11963/1002-7807.lhzsj.20170818

http://journal.cricaas.com.cn/Jweb_mhxb/CN/Y2017/V29/I5/437

参考文献

[1] 邱志坚, 刘文哲. 棉花色素腺体研究进展[J]. 鲁东大学学报(自然科学版), 2008, 24 (2): 162-167.
Qiu Zhijian, Liu Wenzhe. Advances in research on pigment gland of cotton[J]. Ludong University Journal (Natural Science Edition), 2008, 24(2): 162-167.
[2] 季道藩. 棉花知识百科[M]. 北京: 中国农业出版社, 2001: 23- 25.
Ji Daofan. Cotton knowledge encyclopedia[M]. Beijing: China Agriculture Press, 2001: 23-25.
[3] 姚军, 何晓文, 李新霞, 等. 高效液相色谱-质谱法测定棉籽仁中左旋和右旋棉酚含量[J]. 食品工业科技, 2013, 34(19): 277- 280.
Yao Jun, He Xiaowen, Li Xinxia, et al. Determination of (-)- and (+)-gossypol in cottonseed by high-performance liquid chromatography with mass spectrum[J]. Science and Technology of Food Industry, 2013, 34(19): 277-280.
[4] Adams R, Geissman T A, Edwards J D. Gossypol, a pigment of cottonseed[J]. Chemical Reviews, 1960, 60(6): 555-574.
[5] Bell A A. Formadtion of gossypol in infected or chemically irritated tissues of Gossypium species[J]. Phytopathology, 1967, 57: 759-764.
[6] 季道藩. 棉花腺体的遗传和育种[J]. 浙江农业大学学报, 1980, 6(1): 32-45.
Ji Daofan. The genetic and breeding for cotton pigment glands[J]. Journal of Zhejiang Agricultural University, 1980, 6(1): 32-45.
[7] 何晓文, 李新霞, 姚军, 等. 柱前手性衍生化-高效液相色谱法测定棉籽仁中左旋和右旋棉酚含量[J]. 食品科学, 2013, 34(12): 125-128.
He Xiaowen, Li Xinxia, Yao Jun, et al. Determination of (-) and (+)-gossypols in cottonseed kernel by high-performance liquid chromatography with chiral pre-column derivatization[J]. Food Science, 2013, 34(12): 125-128.
[8] 邓德旺. 棉花色素腺体的研究进展[J]. 河北农业大学学报, 1988, 11(4): 125-131.
Deng Dewang. Advance of research on the pigment gland of cotton[J]. Journal of Agricultural University of Hebei, 1988, 11(4): 125-131.
[9] 沈仍愚, 陈铭荻, 王新其, 等. 估测棉仁中棉酚含量研究续报[J]. 中国棉花, 1988(3): 23-25.
Shen Rengyu, Chen Mingdi, Wang Xinqi, et al. Estimation of cottonseed gossypol contents in the research report[J]. China Cotton, 1988(3): 23-25.
[10] Smith F H. Determination of gossypol in leaves and flower buds of Gossypium[J]. Journal of the American Oil Chemists Society, 1967, 44(4): 267-269.
[11] 朱四元, 陈金湘. 低酚棉育种研究进展[J]. 江西农业学报, 2006, 18(4): 18-23.
Zhu Siyuan, Chen Jinxiang. Advances in breeding of low gossypol cotton[J]. Acta Agriculturae Jiangxi, 2006, 18(4): 18-23.
[12] 张程程, 吴燕民, 唐益雄. 棉酚及其衍生物合成途径的研究进展[J]. 生物技术进展, 2011, 1(3): 165-171.
Zhang Chengcheng, Wu Yanmin, Tang Yixiong. Progress on biosynthetic pathway of gossypol and its derivatives[J]. Current Biotechnology, 2011, 1(3): 165-171.
[13] 唐爱民, 万长寿. 棉株不同部位棉酚含量及其与表型的相关分析[J]. 中国棉花, 1985(6): 20-22.
Tang Aimin, Wan Changshou. Correlation analysis between gossypol content in different parts and phenotype of cotton[J]. China Cotton, 1985(6): 20-22.
[14] 狄佳春, 陈旭升, 吴巧娟, 等. 棉盲蝽对低酚棉和有酚棉为害的差异[J]. 中国棉花, 2007, 34(11): 20-21.
Di Jiachun, Chen Xusheng, Wu Qiaojuan, et al. Damage differences of cotton fleahopper in glandless cotton and glanded cotton[J]. China Cotton, 2007, 34(11): 20-21.
[15] 宋凤鸣, 郑重. 棉酚和单宁含量与棉花对枯萎病抗性之间的关系[J]. 浙江农业大学学报, 1997, 23(5): 529-532.
Song Fengming, Zheng Zhong. Involvement of gossypol and tannin in the resistance of cotton seedlings to Fusarium wilt[J]. Journal of Zhejiang Agricultural University, 1997, 23(5): 529- 532.
[16] 朱美霞, 李永起, 戴小枫. 棉酚腺体与棉酚生物合成及低酚棉分子育种[J]. 分子植物育种, 2004, 2(3): 307-312.
Zhu Meixia, Li Yongqi, Dai Xiaofeng. Gossypol gland and gossypol biosynthesis and molecular breeding of low gossypol cotton[J]. Molecular Plant Breeding, 2004, 2(3): 307-312.
[17] 代友彪, 杨四涛, 唐辉. 左旋棉酚抗肿瘤作用的研究进展[J]. 中国药业, 2009, 18(19): 1-3.
Dai Youbiao, Yang Sitao, Tang Hui, Advances on anti-tumor research of (-)-gossypol[J]. China Pharmaceutical, 2009, 18(19): 1-3.
[18] 雷海鹏. 棉酚作为男用节育药的前途[J]. 药学学报, 1983, 18(5): 321-324.
Lei Haipeng. Gossypol used as a male contraceptive[J]. Acta Pharmaceutica Sinica, 1983, 18(5): 321-324.
[19] Zhang Mi, Zheng Xuelian, Song Shuiqing, et al. Spatiotemporal manipulation of auxin biosynthesis in cotton ovule epidermal cells enhances fiber yield and quality[J]. Nature Biotechnology, 2011, 29(5): 453-458.
[20] Wilson F D, Smith J N. Some genetic relationships between gland density and gossypol content in Gossypium hirsutum L.[J]. Crop Science, 1976, 16(6): 830-832.