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棉花陆海渐渗系双交分离群体产量和纤维品质性状的QTL定位

孔令磊1,2#,石玉真2#,李邵琦2,黎波涛2,李俊文2,刘爱英2,龚举武2,商海红2,巩万奎2,葛群2,王艳玲2,宋威武2,袁有禄2*   

  1. 1.新疆农业大学农学院,乌鲁木齐 830000;2.中国农业科学院棉花研究所/棉花生物学国家重点实验室/农业部棉花生物学与遗传育种重点实验室,河南 安阳 455000
  • 收稿日期:2017-04-07 出版日期:2018-03-15 发布日期:2018-03-15
  • 通讯作者: yylcri@126.com
  • 作者简介:孔令磊(1991―),女,博士研究生,linglei_kong@foxmail.com。 # 同等贡献。 * 通信作者:yylcri@126.com
  • 基金资助:
    国家自然科学基金(31101188);国家863 计划(2012AA101108);中央级公益性科研院所基本科研业务费专项重点项目(161016201733)

QTL Mapping for Yield and Fiber Quality Traits in Double-Cross Population of Chromosome Segment Introgression Lines from Gossypium hirsutum×Gossypium barbadense

Kong Linglei1, 2#, Shi Yuzhen2#, Li Shaoqi2, Li Botao2, Li Junwen2, Liu Aiying2, Gong Juwu2, Shang Haihong2, Gong Wankui2, Ge Qun2, Wang Yanling2, Song Weiwu2, Yuan Youlu2*   

  1. 1. College of Agriculture, Xinjiang Agricultural University, Urumqi, Xinjiang 830000, China; 2. Institute of Cotton Research of Chinese Academy of Agricultural Sciences/ State Key Laboratory of Cotton Biology/ Key Laboratory of Biological and Genetic Breeding of Cotton, Ministry of Agriculture, Anyang, Henan 455000, China
  • Received:2017-04-07 Online:2018-03-15 Published:2018-03-15

摘要: 【目的】利用海岛棉染色体片段渐渗系对棉花产量和纤维品质相关QTL(Quantitative trait locus)进行定位。【方法】以陆地棉中棉所45和海岛棉海1高代回交自交获得的4个优质海岛棉染色体片段渐渗系MBI 7115、MBI 7412、MBI 7153、MBI 7346为亲本,通过[(MBI 7115×MBI 7412)×(MBI 7153×MBI 7346)]构建双交F1及F1:2群体。利用微卫星标记(Simple sequence repeat,SSR)对亲本及双交F1进行基因型分析,并对F1、F1:2群体的产量和纤维品质相关性状进行QTL定位。【结果】4个渐渗系遗传背景恢复到轮回亲本中棉所45的比例均在97%以上,在2个群体中共检测到41个QTL,分布在11条染色体上。其中与纤维品质相关的有30个,表型贡献率在1.11%~11.80%;与产量相关的有11个,表型贡献率在1.09%~13.57%。【结论】有5个纤维品质相关的QTL能同时在2个群体中检测到且均为新发现的QTL。这一结果为进一步精细定位和开展棉花分子标记辅助育种提供了重要依据。

关键词: 棉花; 染色体片段渐渗系; QTL定位; 产量; 纤维品质

Abstract: [Objective] The aim of this study was to map quantitative traits loci (QTLs) for yield and fiber quality traits in chromosome segment introgression lines (CSILs) from Gossypium hirsutum×Gossypium barbadense. [Method] Four CSILs, MBI 7115, MBI 7412, MBI 7153 and MBI 7346, which were obtained by advanced backcrossing and continuous inbreeding from upland cotton variety CCRI 45 and sea-island cotton variety Hai 1, were used to construct double-cross segregating populations F1 and F1:2 through the following crosses: [(MBI 7115×MBI 7412)×(MBI 7153×MBI 7346)]. Simple sequence repeat (SSR) molecular markers were used to evaluate the genotyes of parents. The F1 and F1:2 populations were used to map QTLs for yield and fiber quality-related traits. [Result] The recovery rates of the recurrent parent CCRI 45 in the four CSILs were all above 97%. Forty-one QTLs, which were distributed across 11 chromosomes, were detected using the two segregating populations. There were 30 QTLs controlling fiber quality with phenotypic variations ranging from 1.11% to 11.80% and 11 QTLs controlling yield-related traits with 1.09%–13.57% phenotypic variations. [Conclusion] Five QTLs for fiber quality were consistently detected in two populations and they were all newly discovered QTLs. This study provides an important theoretical basis for fine mapping of these QTLs and molecular marker-assisted breeding for excellent fiber quality.

Key words: cotton; chromosome segment introgression lines; QTL mapping; yield; fiber quality

中图分类号: 
  • S562.035